Shewanella oneidensis cis-Regulatory Element Database

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Summary of Putative Motif Profiles identified in Shewanella Oneidensis

(The online supplementary material for The cis-regulatory map of Shewanella genomes, NAR 2008)

 

Dataset I

Dataset II

Microarray Dataset

The anchor genome (s)

S. oneidensis and E. coli

S. oneidensis

S. oneidensis

Other genomes      

At least 1 other Shewanella species

At least 2 other Shewanella species

At least 2 other Shewanella species

Number of promoter sets of orthologous genes

862

1961

460

Step I: Phylogenetic footprinting with iterative CONSENSUS search

(Motifs that are conserved across multiple species, redundant)

11247

22525

5355

Step II: PhyloNet Searching

(Motifs that are not only conserved across multiple species, but also shared by different genes, still redundant)

203

1665

337

Step III: Motif hierarchical clustering

(Final non-redundant motifs)

38* (189**) 183 (824)

31 (124)

          Number of motifs matching to known TF binding sites

          6

          9

          4

          Number of motifs with GO/KEGG enrichment

          6

          24

          5

          Number of motifs with expression coherence

          13

          46

          12

          Number of motifs with at least one supporting evidence

          16

          59

          15

* Number of non-redundant motifs ultimately identified;

** Number of TUs covered by the predicted motifs..

Click the links to access the detailed information for different datasets.

 

--- For Bioinformaticians who prefer to parse the results themselves

Download Raw Data (matrix profiles & description files, including the information of site positions in S. oneidensis):

 

Dataset I Dataset_I.tar
Dataset II Dataset_II.tar
Microarray Dataset Microarray_set.tar

 

Example of *.desc file

Shewanella_oneidensis_gi24345641_nm1

Matrix:

width     = 10

tollr     = 72.80

IC        = 11.830

P-value   = 4.97732E-18

Consensus = AGCcTAgGCT

A |   50    2    2    8    6   47   17    0    0    2

C |    0    6   54   33    2    3    0    2   50    6

G |    6   50    2    0    3    2   33   54    6    0

T |    2    0    0   17   47    6    8    2    2   50

Gene Cluster size = 4:

Shewanella_oneidensis_gi24346409    AGCCTAGGCT    -15    12.07    5.915

Shewanella_oneidensis_gi24345641    AGCCTAGGCT    -28    12.07    5.915

Shewanella_oneidensis_gi24346539    AGCTTAGGCT    -39    11.01    5.915

Shewanella_oneidensis_gi24346298    AGCCAAGGCT    -50C (Starting position*)    10.05 (Patser Score)    5.915 (Patser Cutoff)

* The 3'-end promotor starts from -1.  "C" means the motif is on the complementary strand.

 


Summary of Conservation of the known E. coli transcriptional regulatory interactions in S. oneidensis

(Table 2 for The cis-regulatory map of Shewanella genomes, NAR 2008)

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© 2008 Xing Xu, Jiajian Liu and Gary Stormo, Washington University School of Medicine.